Device

Part:BBa_K5452134

Designed by: Bianca Henriques de Oliveira   Group: iGEM24_USP-EEL-Brazil   (2024-09-28)


Overexpression GPPS

this composite is responsible for expressing GPPS through a strong promoter, with the aim of generating greater production of GPP. Composite contains parts BBa_K801011,BBa_K5452107, BBa_J435219, BBa_K5452108. This composite CDS is for use in eukaryotic chassis, and is currently optimized for Saccharomyces cerevisiae. Assembled with no scars, suited for cloning and assembling into plasmids and other parts through Gibson Assembly.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2812
    Illegal EcoRI site found at 4495
    Illegal XbaI site found at 4844
    Illegal SpeI site found at 1474
    Illegal SpeI site found at 5020
    Illegal PstI site found at 1161
    Illegal PstI site found at 3232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2812
    Illegal EcoRI site found at 4495
    Illegal SpeI site found at 1474
    Illegal SpeI site found at 5020
    Illegal PstI site found at 1161
    Illegal PstI site found at 3232
    Illegal NotI site found at 2278
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2812
    Illegal EcoRI site found at 4495
    Illegal BamHI site found at 1254
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2812
    Illegal EcoRI site found at 4495
    Illegal XbaI site found at 4844
    Illegal SpeI site found at 1474
    Illegal SpeI site found at 5020
    Illegal PstI site found at 1161
    Illegal PstI site found at 3232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2812
    Illegal EcoRI site found at 4495
    Illegal XbaI site found at 4844
    Illegal SpeI site found at 1474
    Illegal SpeI site found at 5020
    Illegal PstI site found at 1161
    Illegal PstI site found at 3232
    Illegal AgeI site found at 106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3922
    Illegal SapI.rc site found at 278
    Illegal SapI.rc site found at 1246
    Illegal SapI.rc site found at 3769


Geranyl diphosphate synthase is the enzyme responsible for the conversion of IPP into GPP in the monoterpenoid pathway in S. cerevisiae. GPP is produced exclusively as an intermediate in the synthesis of farnesyl diphosphate, which results in very limited availability of GPP for monoterpene synthesis. However, the impact of GPP pool regulation in S. cerevisiae has not yet been extensively studied. Thus, the overexpression of GPPS in yeast could allow us to both increase GPP production and gain a better understanding of its functioning, which would be useful in various fields.


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